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viral construct aav2 encoding gfp via the cag promoter  (Addgene inc)


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    Addgene inc viral construct aav2 encoding gfp via the cag promoter
    Viral Construct Aav2 Encoding Gfp Via The Cag Promoter, supplied by Addgene inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/viral construct aav2 encoding gfp via the cag promoter/product/Addgene inc
    Average 90 stars, based on 1 article reviews
    viral construct aav2 encoding gfp via the cag promoter - by Bioz Stars, 2026-02
    90/100 stars

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    ( A ) IUE was performed on gestation day 16 (E16) CD01 dams (Cartoon adapted from Phadke et al. (2024). In control conditions, we expressed GFP under the strong CAG promoter. In the C4-OE condition, we electroporated two plasmids under the CAG promoter to express GFP and overexpress mouse C4 ( mC4, C4b, or C4 ). All plasmids were expressed at a final concentration of 1 μg/μl. ( B ) Cartoon and slices of the P21-23 PFC containing cells that have been isolated using microdissection (yellow dotted line). scale bar = 100 μm. ( C ) After microdissection, mPFC tissue was immediately flash-frozen on dry ice. Next, cDNA library preparation was performed by the Broad Genomics Platform using the Illumina TruSeq™ Stranded mRNA Sample Preparation Kit (isolating at least 250 ng of purified total RNA). Whole transcriptome sequencing was performed at the Broad Institute Genomic Services, and R (v4.2.1) was used for further analysis.

    Journal: bioRxiv

    Article Title: Increased Complement C4 in a Sparse Neuronal Subset Induces Network-Wide Transcriptomic Alterations in the Prefrontal Cortex

    doi: 10.1101/2025.05.29.656749

    Figure Lengend Snippet: ( A ) IUE was performed on gestation day 16 (E16) CD01 dams (Cartoon adapted from Phadke et al. (2024). In control conditions, we expressed GFP under the strong CAG promoter. In the C4-OE condition, we electroporated two plasmids under the CAG promoter to express GFP and overexpress mouse C4 ( mC4, C4b, or C4 ). All plasmids were expressed at a final concentration of 1 μg/μl. ( B ) Cartoon and slices of the P21-23 PFC containing cells that have been isolated using microdissection (yellow dotted line). scale bar = 100 μm. ( C ) After microdissection, mPFC tissue was immediately flash-frozen on dry ice. Next, cDNA library preparation was performed by the Broad Genomics Platform using the Illumina TruSeq™ Stranded mRNA Sample Preparation Kit (isolating at least 250 ng of purified total RNA). Whole transcriptome sequencing was performed at the Broad Institute Genomic Services, and R (v4.2.1) was used for further analysis.

    Article Snippet: For GFP control, we used a plasmid containing EGFP under the CAG promoter (pCAG-EGFP, Addgene plasmid #11150; http://n2t.net/addgene:11150;RRID:Addgene_11150 ) ( ).

    Techniques: Control, Concentration Assay, Isolation, Laser Capture Microdissection, cDNA Library Assay, Sample Prep, Purification, Sequencing